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KMID : 0368420060490040298
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Journal of Plant Biology
2006 Volume.49 No. 4 p.298 ~ p.302
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Analysis of gene expression upon DNA damage inArabidopsis
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Kim Youn-Sung
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Abstract
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To understand their responses to DNA damage,Arabidopsis plants were exposed to methyl methanesulfonate (MMS). Such treatment inhibited growth and decreased the leaf chlorophyll content. A concomitant change in the expression of cell cyclerelated genes also occurred.CYCB1 expression was slightly increased but that ofCYCD1 declined. To identify the genetic elements in these responses, genome-wide transcription profiling ofArabidopsis was performed following the MMS treatment, using a cDNA microarray. Expression was altered by more than two-fold for 3666 genes ? i.e., 1657 genes showed an increase and 2009 genes had a decrease in transcripts. Five DNA repair-related genes were slightly enhanced, while several disease resistance-related and glutathione transferase genes were strongly up-regulated. Interestingly, 27 ubiquitin-related genes were also altered by more than two-fold, suggesting that protein degradation may have been involved in those damage responses. These microarray results were validated by RT-PCR.
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KEYWORD
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Arabidopsis, DNA damage, methyl methanesulfonate, microarray
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